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细胞分离磁性粒子对紫外吸收分光光度法定量 DNA 的影响
Izza Usman Bajwa 1 , Samuel Sigaud 1* 1 Accumol Inc.，加拿大艾伯塔省卡尔加里 * samuel.sigaud@accumol.com 摘要 磁性粒子通常用于从血液样本中分离特定类型的细胞。从这些细胞中提取的基因组 DNA 中的残留粒子会干扰紫外吸收分光光度法的浓度测量。在本研究中，我们在谱系特异性嵌合体分析工作流程中确定了紫外分光光度法 DNA 定量的不准确程度。我们发现残留磁性粒子和 RNA 的存在会导致对 DNA 浓度的估计过高。简介使用磁性粒子从血液样本中分离特定类型细胞是诊断或免疫遗传学实验室的常用技术。例如，谱系特异性嵌合体分析的典型工作流程包括从血液样本中分离 T 淋巴细胞、髓细胞或其他细胞类型，然后提取基因组 DNA，然后进行 PCR 或 qPCR 1 。提取后通常会检查 DNA 浓度和质量，以确保下游 PCR 反应在最佳条件下进行。根据 DNA 提取方法，在最终 DNA 样本中可能会发现用于细胞分离步骤的残留磁性粒子。虽然这些粒子通常不会干扰后续的 PCR 反应，但它们可能会影响 DNA 定量步骤。紫外吸光度分光光度法是评估 DNA 浓度和纯度最广泛的方法。它速度快，不需要使用标准曲线或特殊试剂。它使用非常少量的 DNA，尤其是使用无比色皿分光光度计（如 NanoDrop 仪器（ThermoFisher Scientific））进行时。然而，紫外吸光度对 DNA 2 不具有选择性。浓度测量可能会受到污染物的影响，例如 RNA、蛋白质、DNA 提取过程中使用的化学品或用于细胞分离的磁性粒子。为了克服这些问题，已经开发出荧光 DNA 结合染料 3。这些化合物与双链 DNA 结合时会显著增强荧光。它们具有高度的特异性和灵敏度，现在被认为是 DNA 定量的黄金标准。然而，与紫外分光光度法相比，荧光测量更耗时，需要使用昂贵的试剂，并需要实现 DNA 标准曲线。由于这些原因，当许多样本需要快速处理时，例如在分子诊断实验室中，紫外分光光度法仍然是确定 DNA 浓度的首选方法。本研究的目的是确定在谱系特异性嵌合体分析工作流程中紫外分光光度法 DNA 定量的不准确程度。我们研究了残留磁粒子对 DNA 浓度和质量测量的影响，并提出了提高测量准确性的建议。
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名字 姓氏 提供商输入的企业名称 代理机构名称 企业地址 企业郊区 企业所在州 企业所在国家 企业电话 电子邮件地址 MARA 代理 ID 代理类型 SUKHDEV SINGH SUKHDEV & AHMAD MIGRATION PTY LTD SA Migration Pty Ltd Level 5, Suite 505, 235 Queens street Melbourne VIC Australia 0425043399 education@samigration.com.au 提供商创建的代理 Pankul Gandhi Advanced Migration Pty. Ltd. Advanced Migration 14 GREENSTEAD WAY, CAIRNLEA, VIC 3023 AUSTRALIA CAIRNLEA VIC Australia +61470314456 info@advancedmigration.com.au 1688115 提供商创建的代理 Nishan Singh Agam Migration and Education Consultant AGAM GROUP Education & Migration Consultants Level 1 Suite 5, 16 Main Street Blacktown NSW Australia 0296716133 n.singh@agammigration.com.au 提供商创建代理 Shubham Aggarwal Aggarwal Consultants PTY LTD Aggarwal Consultants PTY LTD 31 Holyoake Parade Wyndham Vale VIC 澳大利亚 433582001 infoaggarwal3@gmail.com 1791186 提供商创建代理 AKASH SANDHU Akash Immigration Sandhu Education 4 Greenglade Way Cranbourne East VIC 澳大利亚 0411 109 286 akashsandhu@outlook.com.au 提供商创建代理 Edmund Galvez AMVPS 澳大利亚 EDMUND SOLEIL GALVEZ Room 28, Level 14, 330 Collins St Melbourne VIC 澳大利亚 0414709110 Amvpsaustralia@gmail.com 1909251 提供商创建代理 Mohammad Saghir Khan 澳大利亚 -东盟教育与移民服务 澳大利亚-东盟教育与移民服务 411 室，4 楼，365 Little Collins Street Melbourne VIC Australia 03 9994 7935 s.khan@ozaseaneducation.com 提供商创建代理 Jujhar Bajwa Bajwa 移民顾问 Lyallpur Family Trust 的受托人 1002, L 10, 125 Swanston Street Melbourne VIC Australia 0390770507 admissions@bajwaimmigration.com 0742209 提供商创建代理 Chandy LORN Baysis PTY LTD BAYSIS MIGRATION SERVICES 409 室，365 Little Collins Street Melbourne VIC Australia +61 3 9077 8600 admin@baysis.com.au 提供商创建代理 Bhumika Dewan BN 全球咨询服务 BN 全球咨询服务 13/ 200 Queen street Melbourne VIC 澳大利亚 0402652084 australia@globalcareerabroad.com.au 供应商创建的代理人 Deepa Malik CNINE Migration CNINE Migration Level 3, 480 Collins Street, Melbourne VIC 3000 Melbourne VIC VIC 澳大利亚 0433352066 info@cnine.com.au 供应商创建的代理人 Amit Wadhwa DEL2MEL Pty Ltd T/A Study directions Study Directions 401, Level 4, 530 Little Collins Street MELBOURNE VIC 澳大利亚 04 0615 3388 amit@studydirections.com 供应商创建的代理人 Gautam Kanda EAST WEST Education ALLIANCE EAST WEST Education ALLIANCE 92 RIBBLESDALE AV WYNDHAM VALE VIC 澳大利亚 0424809454 info@eastwesteducationalliance.com.au 供应商创建的代理人 Harsatbir Singh独家移民服务独家移民集团 86 Newgrange Bld Clyde QLD 澳大利亚 404924658 info@exclusivemigrationgroup.com。au 供应商创建代理 Sandeep Shoran Gauranga Education & Migration Services Pty Ltd Gauranga Education & Migration Services Pty Ltd 34 Montvale road Truganina VIC Australia 0422 267 921 education@gaurangamigration.com.au 1909915 供应商创建代理 Saurabh Smar Global Vision Migration consultants Global Vision Migration consultants Bourke street 227 Collins street Melbourne VIC Australia +61 430 081 804 info@mygvm.com.au 供应商创建代理 Gagandeep Singh HOLISTIC MIGRATION SOLUTIONS PTY LTD HOLISTIC MIGRATION SOLUTIONS PTY LTD LEVEL 1, SUITE 1 23 KOORNANG ROAD CARNEGIE VIC Australia 0399399036 kamal@holisticgroup.com.au 供应商创建代理 Imran Warraich Huk Legal Services Pty Ltd Huk Legal Services Pty Ltd 217/530 Little Collins St Melbourne VIC Australia 0427 786 444 inwarraich@gmail.com 提供商创建代理人 Shubhangi 印度澳大利亚教育中心 (IAEC) 印度澳大利亚教育中心 (IAEC) Level 9, 267 Collins Street Melbourne VIC Australia 0396701140 admissions@iaec.com.au 提供商创建代理人 SUKHDEV SINGH ION Education CONSULTANTS PTY LTD ION Education CONSULTANTS PTY LTD SUITE G/ 88 MERIVALE STREET SOUTH BRISBANE QLD Australia 0473059766 ADMISSIONS@IONCONSULTANTS.COM.AU 2318018 提供商创建代理人 Preeti Kaur Kaur Migration Pty Ltd Kaur Migration Level 6, 530 Little Collins Street, Melbourne VIC Australia 0390880229 info@kaurmigration.com.au 供应商创建代理 Chaman Preet 移民和教育专家 (MEE) 移民和教育专家 (MEE) 251 High street cbd VIC 澳大利亚 0399957673 admin@mee.org.au 供应商创建代理 Amber Gupta MIGRATION CONSULTANTS- MELBOURNE PTY LTD 墨尔本移民顾问和律师 Suite 609, Level 6, 55 Swanston Street MELBOURNE VIC 澳大利亚 61 3 96633869 melbourne.migration@gmail.com 0533773 移民代理 Duti Singh Migration Intelligence MIGRATION INTELLIGENCE 420, level 2 collins street Melbourne VIC 澳大利亚 0425 085 887 info@migrationintelligence.com 供应商创建代理 Dildeep Singh Milestone 教育与移民服务 MILESTONE 教育与移民服务私人有限公司Suite 3/Level 5 342 Flinders Street Melbourne VIC Australia 0426267476 info@milestonee.com 1281067 供应商创建的代理人 Rakesh Kumar Millennium Career Consultants Pty Ltd Aussie New Zea Migration & Education Consultancy 18 Rodney Avenue Ingle Farm VIC Australia 0423 032 115 admissions@mccvisas.com 供应商创建的代理人 Vijay Sharma Millennium Consultants Millennium Consultants 46 Endeavour Street Seven hills NSW Australia 0435267305 sydney.millennium@gmail.com 供应商创建的代理人 Shiva Neupane NEW PAGE INTERNATIONAL PTY LTD New Page International Pty Ltd Suite 106, 365 Lt Collins St Melbourne VIC Australia 61 9973 6060 info@newpageau.com 供应商创建的代理人 CHANDER BHAN Noble移民和教育专家 Noble Immigration 77 Alarah Bvd Cranbourne West VIC 澳大利亚 0452225206 info@nobleimmigration。au 2318045 供应商创建代理 Gurdevjit Singh Noble Visa Pty Ltd Noble Visa Pty Ltd 36 Shelton Cres NOBLE PARK NORTH VIC 澳大利亚 +61 3 8752 2978 info@noblevisa.com.au 2016337 供应商创建代理 Harpreet Singh Oberoi OBCO Migration & Educational Consultants OBCO Migration & Education Consultants 124-A, Green Park, OPP - ISBT Gate No - 6 Jalandhar Punjab 印度 +91 181 4631487 info@obco.com.au 供应商创建代理 Ravi Turan OM Consultants - Education & Migration Services OM Education & Migration Services 100 Herbert street Northcote VIC 澳大利亚 042 788 3368 info@omconsultants.com.au 供应商创建代理 Namal Fernando Oz Visas Pty Ltd Oz Visas Pty Ltd 31, High View Road Balwyn VIC 澳大利亚 0420403654 ozvisasmel2@gmail.com 提供商创建代理 Rohit Gahlawat PR Education Solutions PR Education SOLUTION PTY LTD 201/110 Church Street Parramatta NSW 澳大利亚 0402027402 info@predusolution.com.au 提供商创建代理 Ayushi Sharma Prime visa Prime Visa 70 Saltlake Blvd Wollert VIC 澳大利亚 0414 060 894 admissions.primevisa@gmail.com 提供商创建代理 Pargat Singh Boparai Rightway Migration & Education Rightway Migration & Education 24 Norman Drive Cornubia QLD 澳大利亚 info@rightwaymigration.com.au 1574012 提供商创建代理 Renante Matobato RM Solicitors RM Solicitors 17 Malmaison Crescent Truganina VIC澳大利亚 0414 868 953 ringo@rmsolicitors.net.au 1910623 提供商创建代理 Raman Brar Safeway Consultants Safeway Immigration Consultants Pvt. Ltd. Gobina Tower Faridkot Punjab 印度 +91 95010 70025 safewayjashan@gmail.com 提供商创建代理 Navjot Singh Savie Migration Education and Citizenship services pty ltd Savie Migration Education and Citizenship Services 5 Upwey Ave Springvale VIC 澳大利亚 0450 272 807 saviemec@gmail.com 1801652 提供商创建代理 Manish Paulgarg PAUL Garg Shri Krishna Education And Immigration Consultants (P) Ltd Shri Krishna Education & Immigration Consultants Opposite Jila Udyog Centre nera sunrise hotel,Main Sirhind Road PATIALA Punjab 印度 919815965854 manishpalgarg@rediffmail.com 0852617 移民代理 Suman Deuja SIENNA DEUJA PTY. LTD 以 GENUINE Education AND VISA SERVICES 的名义开展业务 SIENNA DEUJA PTY. LTD 以 GENUINE Education AND VISA SERVICES 的名义开展业务 28 楼，303 Collins Street Melbourne VIC Australia 03 6978 9066 genuineeducation@gmail.com 提供商创建代理 Priyanka SMG Group Pty Ltd SMG group PTY LTD 8 Boulderwood Way ,Wyndham Vale VIC 3024 Wyndham Vale VIC Australia 0469337251 priyankav90g@gmail.com 2217802 提供商创建代理 Surinder Singh Partola Solution Mode Consultancy Pty Ltd SOLUTION MODE CONSULTANCY PTY. LTD. 38 套房，1 楼 93 Wells Road CHELSEA HEIGHTS VIC 澳大利亚 0425 724 854 sandy.partola@solutionmode.com 0958069 提供商创建代理 BHAVYA MOTWANI SPLENDOR MIGRATION GROUP Splendor Migration Group Suite 2.04 2 楼 Collins Street VIC 澳大利亚 +61414136354 admissions@splendormigrationgroup.com 供应商创建代理 Komal Karkra Spot on Global Spot on Global 9/3442 Pacific Highway Springwood QLD Australia 0424929933 imfo@spoton.global 1802544 供应商创建代理 Mandeep Singh STAR MIGRATION AND Education CONSULTANTS(SMEC) STAR MIGRATION AND Education CONSULTANTS(SMEC) 21 Stanhope Road Tarneit VIC Australia 0403608872 starmigrationoz@gmail.com 供应商创建代理 Raj Kumar Saini Success Overseas Education & Consulting Pty Ltd Success Overseas Education & Consulting Suite 15, 7-9 Leeds Street Footscray VIC Australia 0383547967 info@successtomigration.com.au 供应商创建代理 SHARANPREET KAUR Success Visa Kansal, Amit (Success Educational Consultants) Suite 417 - 418, 48 George Street Parramatta NSW 澳大利亚 +61468791726 admissions@successvisa.com.au 9900625 供应商创建的代理 Tejinder Kalra Tejinder Kalra AUST AM NEWZ 学习和移民顾问 Collins Square, Tower 4, Level 17, 727 Collins Street Docklands VIC 澳大利亚 0414266896 tej.migration@gmail.com 供应商创建的代理 Prince Teona Upright Migration Consultants Upright Migration Consultants Level 5, Suite 7, 342 FLinders Street Melbourne VIC 澳大利亚 +61 432 982 061 admission@uprightmigration.com.au 1571524 供应商创建的代理 Prakash Kiran Kandel Visa Direct 移民和教育服务 Visa Direct 移民和教育服务有限公司 Level 3,Suite 301,530 Little Collins Street Melbourne VIC Australia 0401318612 kiranvisadirect@gmail.com 提供商创建代理 Harjeet Singh Visa Explore Migration and Education Services Visa Explore Migration and Education Services 14 Havelock Lane Mawson Lakes SA Australia 0430033255 harjeet@visaexplore.com 提供商创建代理 Renu Vashist Vision Migration Consultants Pty Ltd Vision Migration Consultants Suite 702, Level 7, 2 Queen Street Melbourne VIC Australia 433700256 visionmigration@gmail.com 提供商创建代理 Deepak Garg Vision Oz Pty Ltd Vision Oz Pty Ltd 4/36 Harris Street HARRIS 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Vision Migration Consultants Suite 702, Level 7, 2 Queen Street Melbourne VIC 澳大利亚 433700256 visionmigration@gmail.com 提供商创建代理 Deepak Garg Vision Oz Pty Ltd Vision Oz Pty Ltd 4/36 Harris Street HARRIS PARK NSW 澳大利亚 garg7610@yahoo.com 提供商创建代理 Parminder Singh Wales Overseas Consultants Wales Overseas Consultants 1004, Level 10, Swanston street Melbourne VIC 澳大利亚 399396830 info@walesoverseas.com 提供商创建代理 NITI HARA WAY2OZ MIGRATION SERVICES PTY LTD WAY2OZ MIGRATION SERVICES PTY LTD Exchange Tower, Level 1,, Lt Collins Street VIC 澳大利亚 info@way2oz.com 1801532 供应商创建代理 Danilo Lopes West1 West1 Suite 9A/ 243 Edward Street, Brisbane, QLD 4000 Brisbane QLD 澳大利亚 0402129045 camila@west1.com.au 供应商创建代理 Parminder Singh Pabla WINGS MIGRATION WINGS MIGRATION 2/9 Henry Street TULLY QLD 澳大利亚 61 4 34199743 pabla.parminder@gmail.com 1468255 移民代理全球 1802544 供应商创建代理 Mandeep Singh STAR MIGRATION AND Education CONSULTANTS(SMEC) STAR MIGRATION AND Education CONSULTANTS(SMEC) 21 Stanhope Road Tarneit VIC 澳大利亚 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