PCR “strip” tubes ....................................................................................................................... 9 PCR 96-well plates ..................................................................................................................... 9 PCR 384-well plates ................................................................................................................... 9 Examples for sample and primer submission ................................................................................. 9 DNA S AMPLE P REPARATION .............................................................................................................. 14 P RIMER P REPARATION ..................................................................................................................... 16
Important Notes 14 Working with QIAwave products 14 Water quality used for preparation of functional buffers 15 Glassware 15 Labeling of functional buffers in glass bottles 15 Waste Tubes 16 Elution tubes 16 Recycling information 16 Sample collection and storage 17 Starting amounts of samples 17 Maximum amount of starting material 17 Very small sample sizes 18 Quantification of starting material 19 Preparation of Buffer AW1, Buffer AW2, and Buffer AE 21 Buffer AL 22 Proteinase K 22 RNA的同份23纯核酸的洗脱23预期产生25纯化高分子量DNA 26
• Elevators/Stair Lifts • Feeding Tubes and Pumps • Hospital Beds/Air Mattresses • Infusion Pumps • Intravenous Pumps (IVs) • Medical Refrigeration • Nebulizers • Oxygen Concentrators • Power Wheelchairs/Scooters • Suctioning Devices • Vacuum-assisted closure (VAC) • Ventilators • _______________________________________ • __________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________]
纤维锂离子电池(FLIBS)对可穿戴电子设备供电。但是,它们的实际应用受到有限的周期和日历寿命的阻碍,这主要是由于通过封装层渗透而导致的活跃LI损失。为了应对这一挑战,将低渗透性和高灵性四氟乙二醇六烷基共聚物共聚物(FEP)管提出,以通过熔化挤出法连续封装FLIB。由于氟氨酸树脂的固有疏水性和聚合物基质的适当结晶度,FEP管表现出明显低的蒸气渗透性,水蒸气透射率(WVTR)为0.3 mg·day -day -day -day -1·Pkg - 1·PK -PK -PK -1,15倍(4. 3倍)(4.6)。 1·PKG - 1)。Leveraging the low permeability and elastic modulus of FEP tubes, FLIBs demonstrate a capacity retention of 80.05% after 180 cycles and exceptional flexibility with a capacity retention of 98.32% after 10 000 bending cycles, showcasing superior performance compared to the conventional polymer tubes (for example, the capacity of PP-FLIBs declined by 20.68% after 30周期)。这项工作提出了一种一般且有效的策略,用于连续封装FLIB,有效地延长了FLIB的循环和日历寿命,从而增强了其可穿戴电子应用的实际生存能力。
•Purecol®(CAT#5005)•干细胞•具有血清和抗生素的细胞培养基•细胞培养瓶•胰蛋白酶-EDTA(Fisher Scientific,Cat#25-200-114)•地塞米松(Dex)(Dex)(Sigma-Aldrich,CAT#D2915-100MG,CAT) -Aldrich,CAT#i5879-5g)•印霉素(IM)(Sigma -Aldrich,CAT#i7378-25g)•4%多羟基甲醛(Thermofisher,CAT#047392-9M) 0.22 µm过滤器•DI水•血细胞•细胞培养板•血清学移液器•Eppendorf Tubes
该疾病的遗传原因是PPIB基因(肽基丙基异构酶b)中的突变,该突变编码了负责胶原蛋白产生的环氨酸B蛋白。这是由PPIB基因115中的错义突变引起的一种常染色体隐性疾病,导致用阿环蛋白替换甘氨酸。需要进行分子诊断,动物DNA提取,PCR(聚合酶链反应)和测序。由于获得的材料是通过动物的最大材料,因此测试了两种头发提取方案。Initially, the hair with bulbs were inserted into Micro Centrifuga tubes with ATL buffer, DTT and proteinase K solution, resulting in a liquid with saponifying, oxidizing and protein solvent properties, capable of dissolving membranes, oxidizing disulfide bridges and breaking down proteins around the genetic material, such as the histons, without damaging the DNA.Initially, the hair with bulbs were inserted into Micro Centrifuga tubes with ATL buffer, DTT and proteinase K solution, resulting in a liquid with saponifying, oxidizing and protein solvent properties, capable of dissolving membranes, oxidizing disulfide bridges and breaking down proteins around the genetic material, such as the histons, without damaging the DNA.
neb零件编号成分描述批号单个QC结果T3062-1MONARCH®蛋白分离解决方案10265475 PASS T3061-1-1-1-莫纳奇®HMWGDNA组织裂解缓冲区10265472 Pass T3056-1Monarch®GDNAELUTION®GDNAELUTIO GDNA洗涤缓冲10265355 PASS T3005-1MONARCH®DNA捕获珠(100)10265326 PASS T3004-1MONARCH®BEADERAINERS10265323 PASS T3003-1管(50)10152756 Pass T2118-1Monarch®SpinCollection Tubes 10245009 Pass P8200SVIAL蛋白酶K,分子生物学级10262750 Pass
气体检测管和手动泵 霍尼韦尔比色气体检测管可快速、现场测量各种有毒和可燃气体和蒸汽。霍尼韦尔检测管易于读取,并可直接根据气体浓度提供清晰、鲜明的颜色变化。霍尼韦尔检测管与霍尼韦尔活塞手动泵兼容,后者是一种可靠的方法,可抽取精确体积的样本,以实现最准确的测量。
-1000 µl带过滤器的1000 µl尖端-100 µl带过滤器的尖端-50毫升管:准备等分试样-5 ml管:每8个样品1个样品制备核量b -b-珠和MWA2混合物 - 2 ml管-2 ml管:1个样品以每样品 + 2转移裂解液以每样样品来制备Elyquots -1.5 ml lock local lock local lock lock locke loce loce luu dna -forse lu dna -forse lu dna -forse luer dna -frus luer dna -luer luer luer luer luer luer luer luer luer luer 96孔板,带2毫升深孔,u底(Macherey Nagel -746032.Deep):每16个样品1-磁杆盖磁盘32(Macherey Nagel 32
Halsic-R 重结晶碳化硅 (RSiC) • 工作温度高达 1600°C(氧化),高达 2000°C(惰性气体)• 高抗热震性 • 高耐腐蚀性 • 标准应用:高温应用的窑具以及气相温度测量管 • 标准几何形状:板、梁、支架、管、保护管、滚筒、匣钵、坩埚、燃烧器喷嘴;可根据要求定制尺寸