葡萄酒微生物群落建立了复杂的生态系统,调节香气化合物的形成,但只有少数研究寻求特定微生物与葡萄酒挥发性物质之间的相关性。本研究结合了代谢条形码和代谢组学,以识别与杜罗河标志性地区 3 个著名品种的葡萄酒挥发性特征相关的真菌和细菌微生物生态位。在整个自然发酵过程中,鉴定了三个主要的微生物生态位,并且 Hanseniaspora - Saccharomyces 的演替时间取决于品种。最大的生态位包括 Hansenias pora、Aureobasidium、Alternaria、Rhodotorula、Sporobolomyces、Massilia、Bacillus、Staphylococcus 和 Cutibacterium,它们与 7 种代谢物呈正相关,即乙偶姻、乙酸异戊酯、丙酸乙酯、c-3-己烯醇、苯乙醚和 4-乙基苯酚。发酵酵母S. cerevisiae、Torulaspora delbrueckii和Meyerozyma caribbica与γ-丁内酯、t-威士忌内酯、异戊醇、癸酸乙酯、异丁酸乙酯、琥珀酸二乙酯、异戊酸、4-乙基愈创木酚和4-丙基愈创木酚呈强相关性。 Lachancea quebecensis 与几种致病真菌(青霉菌、白粉病菌、核盘菌、曲霉菌、Mycosphaerella tassiana)和细菌(假单胞菌属、酸拟杆菌、泛菌、Steno trophomonas 和 Enhydrobacter)聚类,与各种单萜醇和降异戊二烯类化合物(包括芳樟醇和 β-紫罗兰酮)呈正相关,此外还与苯甲醇、二乙酰、乙酸异丁酯、乙基香草酸酯和甲基香草酸酯呈正相关。代谢物-微生物群相关性表明品种特异性可能是区域芳香特征的基础。
1 laqv-requin, department of chemistry, University of Aveiro, 3810-193 Aveiro, Portugal 2 Citab Inov4Agro-Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production, University of T-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal 3 Department of Zootechnics, Ol of Sciences and Technology, University of is Vora, 7000-812 is Vora, Portugal 4 Comprahensive Health Research Center, 7000-812 is Vora, Portugal 5 Engineering Department, University of T-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal 6 Institute for Systems and Computer Engineering, Technology and Science, 4200-465 PORTO, Portugal 7 Animal and Veterinary Research Center (CECAV), University of Tr to S-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal 8 Department of Veterinary Sciences, University of Tr to S-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal * correspondence: pamo@utad.pt1 laqv-requin, department of chemistry, University of Aveiro, 3810-193 Aveiro, Portugal 2 Citab Inov4Agro-Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production, University of T-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal 3 Department of Zootechnics, Ol of Sciences and Technology, University of is Vora, 7000-812 is Vora, Portugal 4 Comprahensive Health Research Center, 7000-812 is Vora, Portugal 5 Engineering Department, University of T-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal 6 Institute for Systems and Computer Engineering, Technology and Science, 4200-465 PORTO, Portugal 7 Animal and Veterinary Research Center (CECAV), University of Tr to S-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal 8 Department of Veterinary Sciences, University of Tr to S-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal * correspondence: pamo@utad.pt
1兽医科学系,农业与兽医科学学院(ECAV),TráS-Os-Montes大学和Alto Douro(UTAD),5000-801 Vila Real,葡萄牙; kamilacgsoares@gmail.com 2兽医和动物研究中心(塞卡夫),TR TO University of t to S-OS-MONTES和ALTO DORO(UTAD),5000-801 Vila Real,葡萄牙3,葡萄牙3,农业和兽医科学学院农学系(ECAV),TR至S-OS-OS-MONTES和ALS-MONTES和ALTO DOURA,UTA,UNTO)。 葡萄牙; cribeiro@utad.pt 4农业环境和生物科学研究与技术中心,农业环境和生物科学研究与技术中心(CITAB)(CITAB),5000-801 VILA REAL,葡萄牙,葡萄牙5000-5000-801 VILA,5000-801 VILA,5000-801 vila vila vila vila vila vila vila 6 Vila Real,葡萄牙 *通信:miguelcastro505@gmail.com(M.C。); Alexutad.pt(A.E.);电话。: +351-914070089(M.C。); +351-917844584(A.E.)
MSM Douro 0019E 05/03/2025 Kattipalli -Tuticorin -10/03/2025 12/03/2025 Tuticorin -Male'
1 CIIMAR – 葡萄牙马托西纽什波尔图大学海洋与环境研究跨学科中心,2 葡萄牙阿威罗大学 UA 生物系,圣地亚哥大学里约分校,阿威罗,3 葡萄牙波尔图大学科学学院 FCUP 生物系,4 葡萄牙维拉雷亚尔特拉奥斯蒙特斯和上杜罗大学 (UTAD) 农业环境与生物科学研究与技术中心 CITAB 生物与环境系,5 西班牙国家研究委员会维哥蓬特韦德拉海洋研究所 IIM-CSIC,6 葡萄牙阿威罗大学 CESAM 环境与海洋研究中心,7 西班牙尼格拉海洋哺乳动物研究协调员 CEMMA,8 西班牙国家研究委员会海洋学系,西班牙维哥蓬特韦德拉
a 里卡多·豪尔赫国立卫生研究所,流行病学系,里斯本,1600-609,葡萄牙 b 特拉什奥斯蒙特斯和上杜罗大学(UTAD),数学系,维拉雷亚尔,5000-801,葡萄牙 c 高等技术学院,数学系,里斯本,1049-001,葡萄牙 d 生物统计学和统计生物信息学跨大学研究所,数据科学研究所,哈瑟尔特大学,比利时 e 卫生经济研究和传染病建模中心,疫苗和传染病研究所,安特卫普大学,比利时安特卫普 f 新国立公共卫生学院,公共卫生研究中心,里斯本新大学,葡萄牙
2风湿病学,庞特·德·利马(Ponte de Lima)Alto Minho的当地卫生部门; 3 Braga Braga医院风湿病学; 4芬加尔医院中心风湿病学医院医院nélioMendonça,Funchal; 5,里斯本圣玛丽亚医院里斯本北医院中心风湿病学; 6风湿病学,Trás-Os-Montes医院中心和Vila Real的Alto Douro; 7风湿病学,Ponta Delgada的DivineEspíritoSanto医院; 8葡萄牙风湿病学院风湿病学研究所,里斯本; 9风湿病学,当地警卫卫生部门,瓜达; 10风湿病学,Vila Nova de Gaia/ Espinho医院中心,Vila Nova de Gaia; 11风湿病,Aveiro Baixo Vouga医院中心; Covilhã的Cova da Beira医院中心风湿病学12; 13 Almada Garcia de Orta医院风湿病学; 14风湿病学,医院De SantoEspíritoda Terceira Island,Angra Do Heroism; 15风湿病学,中间塔加斯医院中心,托马尔
1 Biosystems & Integrative Sciences Institute (Bioisi), Faculty of Sciences of the University of Lisbon, 1749-016 Lisbon, Portugal 2 Grapevine Pathogen Systems Lab, Bioisi School of Sciences of the University of Lisbon, 1749-016 Lisbon, Portugal 3 Department of Vegetable Biology, Faculty of the University of Lisbon, Campo Grande, 1749-0 16 Lisbon, Portugal 4 Quinta dos Murças, spare the Company, Pions, 5050-011 Weight of R is water, Portugal 5 NBI-Natural Business Intelligence, Regia Douro Park, 5000-033 Vila Real, Portugal 6 CE3C-Center for Ecology, Evolution and Environmental Changes & Change-Global Change and Sustainability Institute, science s from the University of Lisbon, Campo Grande, 1749-016 Lisbon, Portugal 7 Department of Que Mica and Bioka, Faculty of Sciences of the University of Lisbon, Campo Grande, 1749-016 Lisbon, Portugal 8 Energy Technologies and Renewable Sorces Department, National Agency for New Technologies, Energy and Sustainable Skirt Research Center, 75026 Rotondella, MT, Italy 9 Mare-Marine and Environmental Sciences Center & Arnet-Aquatic Research Infrastructure Network Associate Laboratory, Faculty of Sciences of the University of Lisbon, Campo Grande, 1749-016 Lisbon, Portugal * correspondence: DIV>1 Biosystems & Integrative Sciences Institute (Bioisi), Faculty of Sciences of the University of Lisbon, 1749-016 Lisbon, Portugal 2 Grapevine Pathogen Systems Lab, Bioisi School of Sciences of the University of Lisbon, 1749-016 Lisbon, Portugal 3 Department of Vegetable Biology, Faculty of the University of Lisbon, Campo Grande, 1749-0 16 Lisbon, Portugal 4 Quinta dos Murças, spare the Company, Pions, 5050-011 Weight of R is water, Portugal 5 NBI-Natural Business Intelligence, Regia Douro Park, 5000-033 Vila Real, Portugal 6 CE3C-Center for Ecology, Evolution and Environmental Changes & Change-Global Change and Sustainability Institute, science s from the University of Lisbon, Campo Grande, 1749-016 Lisbon, Portugal 7 Department of Que Mica and Bioka, Faculty of Sciences of the University of Lisbon, Campo Grande, 1749-016 Lisbon, Portugal 8 Energy Technologies and Renewable Sorces Department, National Agency for New Technologies, Energy and Sustainable Skirt Research Center, 75026 Rotondella, MT, Italy 9 Mare-Marine and Environmental Sciences Center & Arnet-Aquatic Research Infrastructure Network Associate Laboratory, Faculty of Sciences of the University of Lisbon, Campo Grande, 1749-016 Lisbon, Portugal * correspondence: DIV>
