There are no restriction sites for the following enzymes: AarI(x), Acc65I, AcuI, AfeI, AgeI, AlwI, AlwNI, ApaI, AscI, AsiSI, AvrII, BamHI, BanII, BclI, BglI, BglII, BlpI, BmgBI, BmrI, BmtI, BsaI, BsaXI, BsgI, BsmBI, BspDI, BspEI, BsrFI, BsrGI, BstBI, BstEII, BstYI, BstZ17I, Bsu36I, ClaI, DpnI, DpnI, EagI, EcoN, EcoO1 FseI, FspAI(x), HindIII, I-CeeI, I-SceI, CPNI,MBOI,MSCI,NEI,NCOI,NDEI,NGOMIV,NHEI,NENI,NSSII,NSSII,NSPI,PFLI,PFLI,PMMI,PMLI,PMLI,P; PMLI,P; PMLI,P; PMLI,PPUTMI,PPHMI,PPHMI,PPHMI,PPHMI,PPHMI,P; PSPOME,PSPXI,PVI,PVII,RSRII,SACI,SALI,SALI,SANDI(X),SAU3AI,SBFI,SFII,SFII,SFII,SGRI,SGRI,SGRI,SMAI,SMAI,SMAI,SNABI,SPEI,SPEI,SPEI,SPHI,SPHI,SPHI,SRFMI(SRFMI(X)
There are no restriction sites for the following enzymes: AarI(x), Acc65I, AcuI, AfeI, AgeI, AlwI, AlwNI, ApaI, AscI, AsiSI, AvrII, BamHI, BanII, BclI, BglI, BglII, BlpI, BmgBI, BmrI, BmtI, BsaI, BsaXI, BsgI, BsmBI, BspDI, BspEI, BsrFI, BsrGI, BstBI, BstEII, BstYI, BstZ17I, Bsu36I, ClaI, DpnI, DpnI, EagI, EcoN, EcoO1 FseI, FspAI(x), HindIII, I-CeeI, I-SceI, CPNI,MBOI,MSCI,NEI,NCOI,NDEI,NGOMIV,NHEI,NENI,NSSII,NSSII,NSPI,PFLI,PFLI,PMMI,PMLI,PMLI,P; PMLI,P; PMLI,P; PMLI,PPUTMI,PPHMI,PPHMI,PPHMI,PPHMI,PPHMI,P; PSPOME,PSPXI,PVI,PVII,RSRII,SACI,SALI,SALI,SANDI(X),SAU3AI,SBFI,SFII,SFII,SFII,SGRI,SGRI,SGRI,SMAI,SMAI,SMAI,SNABI,SPEI,SPEI,SPEI,SPHI,SPHI,SPHI,SRFMI(SRFMI(X)
saci smai bshii paci sbfi sbfi ecoi访问acci的bamhi xbai xbai xbai sali sali psti psti psti psti hindii agtgaattcgicgtccccccggggggggggggcggcgtcitatatagtagtcightgtgtgtgtgtgtgtgtgtggtggcsggctggctgctctscats。。。。。。。。。。4o
我们报告了一系列适用于检测和克隆翻译控制信号和外源基因 5' 编码序列的质粒载体的构建和使用。在这些质粒中,乳糖操纵子 β-半乳糖苷酶基因 lacZ 的氨基末端的前八个密码子被去除,并在 lacZ 的第八个密码子附近插入独特的 BamHI、EcoRI 和 SmaI (XmaI) 内切酶切割位点。将含有适当调节信号和 5' 编码序列的脱氧核糖核酸片段引入此类 lac 融合质粒导致产生由 β-半乳糖苷酶残基的羧基末端片段和含有外源脱氧核糖核酸序列编码的氨基末端氨基酸的肽片段组成的混合蛋白。这些杂合肽保留了 1,8-半乳糖苷酶的酶活性,并产生了 Lac' 表型。此类杂合蛋白可用于纯化由外源脱氧核糖核酸片段编码的肽序列,以及用于研究特定肽片段的结构和功能。