会员供应商 ID(十进制格式)(AVL) DiTEST Fahrzeugdiagnose GmbH 4621 (Silkland) 深圳市观海科技有限公司 13944 @pos.com 3765 01dB‐Stell 3151 034Motorsport 13566 0XF8 Limited 10737 1010music LLC 13966 103mm Tech 8168 1064138 Ontario Ltd. O/A UNI‐TEC ELECTRONICS 8219 11 WAVE TECHNOLOGY, INC. 4375 1417188 Ontario Ltd. 4835 1C Company 5288 1MORE INC. 12048 28 Gorilla 13617 2D Debus & Diebold Messsysteme GmbH 8539 2L international B.V. 4048 2N 电信公司7303 2‐Tel B.V. 2110 2WCOM GmbH 7343 2Wire, Inc 2248 360 Electrical, LLC 12686 360 Service Agency GmbH 12930 360fly, Inc. 11161 3Brain AG 9818 3C TEK CORP. 9397 3Cam Technology, Inc 1928 3Com Corporation 1286 3D CONNEXION SAM 9583 3D Imaging & Simulations Corp. (3DISC) 11190 3D INNOVATIONS, LLC 7907 3D Robotics Inc. 9900 3D Systems Corporation 10632 3D Technologies Ltd 12655 3DM Devices Inc 2982 3DRUDDER 11770 3DSP 7513 3DV Systems Ltd. 6963 3eYamaichi Electronics Co., Ltd. 8709 3i Corporation 9806 3i techs Development Corp 4263 3layer Engineering 7123 3M 加拿大 2200 3M CMD(通信市场部门) 7723 3M Cogent, Inc. 7717 3M 德国 2597
公司供应商 ID(十进制格式)(AVL)DiTEST Fahrzeugdiagnose GmbH 4621 @pos.com 3765 01dB‐Stell 3151 034Motorsport 13566 0XF8 Limited 10737 103mm Tech 8168 1064138 Ontario Ltd. O/A UNI‐TEC ELECTRONICS 8219 11 WAVE TECHNOLOGY, INC. 4375 1417188 Ontario Ltd. 4835 1C Company 5288 1MORE INC. 12048 2D Debus & Diebold Messsysteme GmbH 8539 2L international BV 4048 2N TELEKOMUNIKACE as 7303 2‐Tel BV 2110 2WCOM GmbH 7343 2Wire, Inc 2248 360 Electrical, LLC 12686 360 Service Agency GmbH 12930 360fly, Inc. 11161 3Brain AG 9818 3C TEK CORP. 9397 3Cam Technology, Inc 1928 3Com Corporation 1286 3D CONNEXION SAM 9583 3D Imaging & Simulations Corp. (3DISC) 11190 3D INNOVATIONS, LLC 7907 3D Robotics Inc. 9900 3D Systems Corporation 10632 3D Technologies Ltd 12655 3DM Devices Inc 2982 3DRUDDER 11770 3DSP 7513 3DV Systems Ltd. 6963 3eYamaichi Electronics Co., Ltd. 8709 3i Corporation 9806 3i techs Development Corp 4263 3layer Engineering 7123 3M 加拿大 2200 3M CMD(通信市场部门) 7723 3M Cogent, Inc. 7717 3M 德国 2597 3M 家庭健康系统 2166 3M 图书馆系统 3372 3M Scott 8463
公司供应商 ID(十进制格式)(AVL)DiTEST Fahrzeugdiagnose GmbH 4621 (Silkland) 深圳市观海科技有限公司 13944 @pos.com 3765 01dB‐Stell 3151 034Motorsport 13566 0XF8 Limited 10737 1010music LLC 13966 103mm Tech 8168 1064138 Ontario Ltd. O/A UNI‐TEC ELECTRONICS 8219 11 WAVE TECHNOLOGY, INC. 4375 1417188 Ontario Ltd. 4835 1C Company 5288 1MORE INC. 12048 28 Gorilla 13617 2D Debus & Diebold Messsysteme GmbH 8539 2L international BV 4048 2N TELEKOMUNIKACE as 7303 2‐Tel BV 2110 2WCOM GmbH 7343 2Wire, Inc 2248 360 Electrical, LLC 12686 360 Service Agency GmbH 12930 360fly, Inc. 11161 3Brain AG 9818 3C TEK CORP. 9397 3Cam Technology, Inc 1928 3Com Corporation 1286 3D CONNEXION SAM 9583 3D Imaging & Simulations Corp. (3DISC) 11190 3D INNOVATIONS, LLC 7907 3D Robotics Inc. 9900 3D Systems Corporation 10632 3D Technologies Ltd 12655 3DM Devices Inc 2982 3DRUDDER 11770 3DSP 7513 3DV Systems Ltd. 6963 3eYamaichi Electronics Co., Ltd. 8709 3i Corporation 9806 3i techs Development Corp 4263 3layer Engineering 7123 3M Canada 2200 3M CMD(通信市场部门) 7723 3M Cogent, Inc. 7717
Company Vendor ID (Decimal Format) (AVL) DiTEST Fahrzeugdiagnose GmbH 4621 @pos.com 3765 01dB‐Stell 3151 034Motorsport 13566 0XF8 Limited 10737 103mm Tech 8168 1064138 Ontario Ltd. O/A UNI‐TEC ELECTRONICS 8219 11 WAVE TECHNOLOGY, Inc。43751417188 Ontario Ltd. 4835 1C公司5288 1More Inc.12048 28 Gorilla 13617 2D Debus&Diebold Messsysteme GmbH 8539 2L国际B.V. 4048 4048 2N TELEKOMUNIKIKACE A.S. 7303 2 -TEL B.V. 2110 2WCOM GMBH 7343 2WIRE,INC 2248 360 ELECTRICAL,LLC 12686 360服务机构服务机构GmbH 12930 360Fly,Inc. 11161 3Brain AG 9818 3C Tek Corp.TekCorp。93973CAM Technology,9397 3CAM Technology,Inciention Corpor Corper corpor corpor corportion&Inimention Corpion Corpion Corpion Corciation&Inmeration Cormitation&Concition 1286 3-33 3-3:SAMN 3-3-3-3-3-3-3-3-3-3 conex&conex& (3DISC)11190 3D Innovations,LLC 7907 3D Robotics Inc. 9900 3D Systems Corporation 10632 3D Technologies LTD 12655 3DM Depplices Inc 2982 3Drudder 11770 3DSP 7513 3DDSP 7513 3DV Systems Ltd. 6963 333 3 Electriation cocoration cocoration cocoration co.909 3 ec.8709090990909090909090909090909. 3i Techs Development Corp 4263 3 Layer Engineering 7123 3M Canada 2200 3M CMD(通信市场部)7723 3M Cogent,Inc。7717 3M德国2597 3M Home Health Systems 2166
公司供应商 ID(十进制格式)(AVL)DiTEST Fahrzeugdiagnose GmbH 4621 (Silkland) 深圳市观海科技有限公司 13944 @pos.com 3765 01dB‐Stell 3151 034Motorsport 13566 0XF8 Limited 10737 1010music LLC 13966 103mm Tech 8168 1064138 Ontario Ltd. O/A UNI‐TEC ELECTRONICS 8219 11 WAVE TECHNOLOGY, INC. 4375 1417188 Ontario Ltd. 4835 1C Company 5288 1MORE INC. 12048 28 Gorilla 13617 2D Debus & Diebold Messsysteme GmbH 8539 2L international BV 4048 2N TELEKOMUNIKACE as 7303 2‐Tel BV 2110 2WCOM GmbH 7343 2Wire, Inc 2248 360 Electrical, LLC 12686 360 Service Agency GmbH 12930 360fly, Inc. 11161 3Brain AG 9818 3C TEK CORP. 9397 3Cam Technology, Inc 1928 3Com Corporation 1286 3D CONNEXION SAM 9583 3D Imaging & Simulations Corp. (3DISC) 11190 3D INNOVATIONS, LLC 7907 3D Robotics Inc. 9900 3D Systems Corporation 10632 3D Technologies Ltd 12655 3DM Devices Inc 2982 3DRUDDER 11770 3DSP 7513 3DV Systems Ltd. 6963 3eYamaichi Electronics Co., Ltd. 8709 3i Corporation 9806 3i techs Development Corp 4263 3layer Engineering 7123 3M 加拿大 2200 3M CMD(通信市场部门) 7723 3M Cogent, Inc. 7717 3M 德国 2597
公司供应商 ID(十进制格式)(AVL)DiTEST Fahrzeugdiagnose GmbH 4621 @pos.com 3765 01dB‐Stell 3151 0XF8 Limited 10737 103mm Tech 8168 1064138 Ontario Ltd. O/A UNI‐TEC ELECTRONICS 8219 11 WAVE TECHNOLOGY, INC. 4375 1417188 Ontario Ltd. 4835 1C Company 5288 1MORE INC. 12048 2D Debus & Diebold Messsysteme GmbH 8539 2L international BV 4048 2N TELEKOMUNIKACE as 7303 2‐Tel BV 2110 2WCOM GmbH 7343 2Wire, Inc 2248 360 Electrical, LLC 12686 360 Service Agency GmbH 12930 360fly, Inc. 11161 3Brain GmbH 9818 3C TEK CORP. 9397 3Cam Technology, Inc 1928 3Com Corporation 1286 3D CONNEXION SAM 9583 3D Imaging & Simulations Corp. (3DISC) 11190 3D INNOVATIONS, LLC 7907 3D Robotics Inc. 9900 3D Systems Corporation 10632 3D Technologies Ltd 12655 3DM Devices Inc 2982 3DRUDDER 11770 3DSP 7513 3DV Systems Ltd. 6963 3eYamaichi Electronics Co., Ltd. 8709 3i Corporation 9806 3i techs Development Corp 4263 3layer Engineering 7123 3M 加拿大 2200 3M CMD(通信市场部门) 7723 3M Cogent, Inc. 7717 3M 德国 2597 3M 家庭健康系统 2166 3M 图书馆系统 3372 3M Scott 8463 3Pea Technologies, Inc. 3637
供应商 ID(十进制格式)(AVL)DiTEST Fahrzeugdiagnose GmbH 4621 @pos.com 3765 01dB-Stell 3151 0XF8 Limited 10737 103mm Tech 8168 1064138 Ontario Ltd. O/A UNI-TEC ELECTRONICS 8219 11 WAVE TECHNOLOGY, INC. 4375 1417188 Ontario Ltd. 4835 1C Company 5288 1MORE INC. 12048 2D Debus & Diebold Messsysteme GmbH 8539 2L international BV 4048 2N TELEKOMUNIKACE as 7303 2-Tel BV 2110 2WCOM GmbH 7343 2Wire, Inc 2248 360 Electrical, LLC 12686 360 Service Agency GmbH 12930 360fly, Inc. 11161 3Brain GmbH 9818 3C TEK CORP. 9397 3Cam Technology, Inc 1928 3Com Corporation 1286 3D CONNEXION SAM 9583 3D Imaging & Simulations Corp. (3DISC) 11190 3D INNOVATIONS, LLC 7907 3D Robotics Inc. 9900 3D Systems Corporation 10632 3D Technologies Ltd 12655 3DM Devices Inc 2982 3DRUDDER 11770 3DSP 7513 3DV Systems Ltd. 6963 3eYamaichi Electronics Co., Ltd. 8709 3i Corporation 9806 3i techs Development Corp 4263 3layer Engineering 7123 3M 加拿大 2200 3M CMD(通信市场部门) 7723 3M Cogent, Inc. 7717 3M 德国 2597 3M 家庭健康系统 2166 3M 图书馆系统 3372 3M Scott 8463 3M 触控系统 1430
公司供应商 ID(十进制格式)(AVL)DiTEST Fahrzeugdiagnose GmbH 4621 (Silkland) 深圳市观海科技有限公司 13944 @pos.com 3765 01dB‐Stell 3151 034Motorsport 13566 0XF8 Limited 10737 1010music LLC 13966 103mm Tech 8168 1064138 Ontario Ltd. O/A UNI‐TEC ELECTRONICS 8219 11 WAVE TECHNOLOGY, INC. 4375 1417188 Ontario Ltd. 4835 1C Company 5288 1MORE INC. 12048 28 Gorilla 13617 2D Debus & Diebold Messsysteme GmbH 8539 2L international BV 4048 2N TELEKOMUNIKACE as 7303 2‐Tel BV 2110 2WCOM GmbH 7343 2Wire, Inc 2248 360 Electrical, LLC 12686 360 Service Agency GmbH 12930 360fly, Inc. 11161 3Brain AG 9818 3C TEK CORP. 9397 3Cam Technology, Inc 1928 3Com Corporation 1286 3D CONNEXION SAM 9583 3D Imaging & Simulations Corp. (3DISC) 11190 3D INNOVATIONS, LLC 7907 3D Robotics Inc. 9900 3D Systems Corporation 10632 3D Technologies Ltd 12655 3DM Devices Inc 2982 3DRUDDER 11770 3DSP 7513 3DV Systems Ltd. 6963 3eYamaichi Electronics Co., Ltd. 8709 3i Corporation 9806 3i techs Development Corp 4263 3layer Engineering 7123 3M Canada 2200 3M CMD(通信市场部门) 7723 3M Cogent, Inc. 7717
杨梅 (Myrica rubra 或 Morella rubra;2n = 16) 所产果实风味独特、营养丰富、经济价值高。然而,先前版本的杨梅基因组缺乏序列连续性。此外,迄今为止,尚无大规模种质资源关联分析检查过决定果实品质性状的等位基因和遗传变异。因此,在本研究中,我们使用 PacBio HiFi 长读长为品种‘早嘉’组装了一个端粒到端粒 (T2T) 无间隙参考基因组。得到的 292.60 Mb T2T 基因组揭示了 8 个着丝粒区域、15 个端粒和 28 345 个基因。这代表着杨梅基因组的连续性和完整性的显著提高。随后,我们对 173 个种质进行了重新测序,鉴定出 6 649 674 个单核苷酸多态性 (SNP)。此外,29 个果实品质相关性状的表型分析促成了全基因组关联研究 (GWAS),该研究鉴定了与 28 种性状显著相关的 1937 个 SNP 和 1039 个基因。在 Chr6:3407532 至 5 153 151 bp 区域上鉴定了一个与果实颜色相关的 SNP 簇,包含两个 MYB 基因(MrChr6G07650 和 MrChr6G07660),这些基因在极端表型转录组中表现出差异表达,与花青素合成有关。一个相邻的、紧密连锁的基因 MrChr6G07670(MLP 样蛋白)包含一个外显子错义变体,经证实可使烟草叶片中的花青素产量增加十倍。这个 SNP 簇可能是一个数量性状基因座 (QTL),它共同调控杨梅果实的颜色。总之,我们的研究提出了一个完整的参考基因组,揭示了一系列与果实品质性状相关的等位基因变异,并确定了可以利用来提高杨梅果实品质和育种效率的功能基因。
试剂部分 # 5X 洗涤缓冲液 10 1X 洗涤缓冲液 11 HPV 包被缓冲液 12 DPBS 和 0.2% TWEEN® 20 (DPBS_0.2T) 13 2N H 2 SO 4 14 0.36NH 2 SO 4 15 柠檬黄溶液 16 293TT 解冻培养基 (293TT TM) 17 293TT 维持培养基 (293TT MM) 18 293TT 冷冻培养基 (293TT FM) 19 70% 乙醇 20 293TT VLP/PsV 转染培养基 (DMEM-TF/DMEM-10A) 21 293TT VLP 转染混合培养基 (DMEM 2%) 用于 Transporter 5 22 293TT VLP 转染混合培养基 (DMEM SF) 用于 PEI 23 DPBS-MGCl 2 10mM A/A (DPBS_MgCl_AA) 24 10% Brij58 25 DPBS/0.8M 盐缓冲液 (DPBS_0.8M) 26 46% OptiPrep 27 27% OptiPrep 28 33% OptiPrep 29 39% OptiPrep 30 293TT 假病毒中和试验培养基 (PBNA_M) 31 1M 硫酸铵 32 HPV VLP 转染裂解缓冲液 33 HPV 假病毒 (PsV) 转染裂解缓冲液 34 DPBS+1%BSA (稀释剂) 35 10% TWEEN® 20 (10_T20) 36 PBS+0.05% TWEEN® 20 (PBS_0.05T) 37 PEI 含5% 葡萄糖 (PEI) 38 DPBS/0.5M 盐缓冲液 (DPBS_0.5M) 39 50 MG Sulfo-NHS 40 DPBS+1% Triton X-100 (DPBS_1%TX) 41 50mM MES 42 组氨酸储存缓冲液 43 鞘液 44 PBST-BSA 缓冲液 (PBST_BSA_PAK) 使用干粉包 45 PBST-BSA 缓冲液 (PBST_BSA) 46 PBS+0.05% TWEEN® 20 (Luminex_Wash) 47 板涂层 48 封闭缓冲液 49 Luminex 珠储存缓冲液 50
