[a] X. Liu博士,U。Aschauer博士刘伯恩3012伯恩(瑞士)的化学,生物化学和药学科学系电子邮件:shi-xia.liu@unibe.chunibe.ch [b] X. Liu化学系Zhejiang Sciech Sciech University hangzzhou(中国)[C] A. Mate.Mate.Mate.Mate.menda,Munem,Munies,Munies,Munies,Munboine,X. Liu博士。 P. Jelinek捷克科学学院物理研究所16200 Prague(捷克共和国)电子邮件:jelinekp@fzu.cz [D] A. Matej,P。Jelinek博士P. Jelinek博士高级技术和材料材料捷克高级技术和研究所(Catrin S.Günther博士,L。L. Patera博士化学与催化研究中心慕尼黑技术大学85748 Garching(德国)电子邮件:laerte.patera@tum.de [F] 和A.M.同样贡献。伯恩3012伯恩(瑞士)的化学,生物化学和药学科学系电子邮件:shi-xia.liu@unibe.chunibe.ch [b] X. Liu化学系Zhejiang Sciech Sciech University hangzzhou(中国)[C] A. Mate.Mate.Mate.Mate.menda,Munem,Munies,Munies,Munies,Munboine,X. Liu博士。 P. Jelinek捷克科学学院物理研究所16200 Prague(捷克共和国)电子邮件:jelinekp@fzu.cz [D] A. Matej,P。Jelinek博士P. Jelinek博士高级技术和材料材料捷克高级技术和研究所(Catrin S.Günther博士,L。L. Patera博士化学与催化研究中心慕尼黑技术大学85748 Garching(德国)电子邮件:laerte.patera@tum.de [F]和A.M.同样贡献。
摘要:RNA编程的CRISPR效应蛋白CAS12A已成为基因编辑和分子诊断的强大工具。但是,需要其他生物工程策略来控制CAS12A活动。在这里,我们表明,可以使用toehold开关DNA发夹,并在环路中呈现一个合理设计的锁定的原始探针相邻基序(PAM),可用于控制CAS12A,以响应分子输入。通过链位移反应从发夹中重新配置Toehold开关DNA从发夹到双链体构象,这提供了一种有效的手段来调节PAM的可及性,从而控制了Cas12a的结合和裂解活性。通过这种方法,我们通过利用基于接近度的链位移反应来响应目标结合来展示触发下游CAS12A活性的潜力。通过利用Cas12a的反式分解活性作为信号转导方法,我们证明了我们的传感应用方法的多功能性。我们的系统可以快速地检测具有高灵敏度和特异性的IgG抗体和小分子。除了生物分析应用外,可开关的PAM设计的脚趾开关还可以作为可编程工具,能够调节基于CAS12A的靶向和DNA处理,以响应分子输入,并且对广泛的生物技术应用持希望。
• Modern Inorganic Molecular and Coordination Chemistry (Sose, 6 ECTS, also profile 1) • Advanced Materials Analysis: Structure and Properties (Sose, 6 ECTS) • Solid State and Materials Chemistry (Sose, 6 ECTS) • Functional Organic Molecules (two -semester) • Liquid Crystals (Wise, only every second year, 6 ECTS) • Polymers materials (SOSE, 6 ECTS) • Modern polymer synthesis (Wise, 6 ECTS, also profile 1) • Structure and Properties of Functional Polymers (SOSE, 6 ECTS) • Polymer Electronics (Wise, 3 ECTS) • Atomic Transportation and Phase Transformation (SOSE, 6 ECTS) • Emulsions & foams (SOSE, 3 ECTS) • Nanoparticles and nanomotors: properties and materials (SOSE, 3 ECTS) • Physical material属性(明智的,仅每二年,6个ECT)•结构分析和材料显微镜(明智,仅每第二年,6年,6个ECTS)先进(SOSE,6个ECT,也是模块容器V)•电化学能量存储(Wise,3 Ect,3 Ects)• Modern Inorganic Molecular and Coordination Chemistry (Sose, 6 ECTS, also profile 1) • Advanced Materials Analysis: Structure and Properties (Sose, 6 ECTS) • Solid State and Materials Chemistry (Sose, 6 ECTS) • Functional Organic Molecules (two -semester) • Liquid Crystals (Wise, only every second year, 6 ECTS) • Polymers materials (SOSE, 6 ECTS) • Modern polymer synthesis (Wise, 6 ECTS, also profile 1) • Structure and Properties of Functional Polymers (SOSE, 6 ECTS) • Polymer Electronics (Wise, 3 ECTS) • Atomic Transportation and Phase Transformation (SOSE, 6 ECTS) • Emulsions & foams (SOSE, 3 ECTS) • Nanoparticles and nanomotors: properties and materials (SOSE, 3 ECTS) • Physical material属性(明智的,仅每二年,6个ECT)•结构分析和材料显微镜(明智,仅每第二年,6年,6个ECTS)先进(SOSE,6个ECT,也是模块容器V)•电化学能量存储(Wise,3 Ect,3 Ects)
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~ 30%:高知名度出版物:Nature materials、Nature energy、Nature physics、Nature chemistry、PRL、Nature Communications、PRX、Advanced materials、Angewandte Chemie International Edition、JACS 等;
27。国际贸易展览会和实验室,测量技术和化学自动化(ILMAC)/国际贸易集市和实验室,测量技术和化学自动化会议的专业会议 div>
1 ICFO—Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, Barcelona, Spain 2 School of Biosciences, Cardiff University, Museum Avenue, CF10 3AX Cardiff, United Kingdom 3 Department of Physics and Materials Science, University of Luxembourg, 162a avenue de la Faïencerie, L-1511 Luxembourg, Luxembourg 4 ICREA,pg。lluís公司公司23,08010西班牙巴塞罗那5 AIX MARSEILLE UNIV,CNRS,CNRS,CENTRALE MARSEILLE,MARSEILLE,INSTITUT FRESELLE,MARSEILLE,MARSEILLE,法国6 Chemie,Chemie,Chemie,Undersitätkonstanz,Konstanz D-78457,德国7点灯光conversationion,Keramiku st。 2B,维尔纽斯LT-10233,立陶宛8物理与天文学学院,加的夫大学,游行,加的夫CF24 3AA,英国9 GSK MEDICINS研究中心,英国Stevenage,英国10号,伊利诺伊州伊利诺伊大学,URBANA-CHAMPEANN,伊利诺伊州Champaign,伊利诺伊州Champairs,Unitais of Unity of Unity of Unity of Unity of Unity of Unity of Unity of Unity of Unity of Unity of Unity of Unity of Unity of Unitiate of Unity of Anime of Unity of Unity of Unity of Anipera Emilia,通过Giuseppe Campi,213/A,Modena I-41125,意大利12号IFN-CNR,Dipartimento di Fisica,Politecnico di Milano,Piazza Leonardo da Vinci 32,I-20133 Milano,I-20133 Milano,Italy 13 Italy 13 Istituto nancien andpien-nansoscien-113 32 I-41125,意大利14 Vilnius University Laser Research Center,Sauletekio AV。10,LT-10223 Vilnius,立陶宛