•尖叫(共识读套件),这是一种新的分子条形码感知的PCR重复工具,用于SUELESELECT XT HS和XT HS2数据,可代替SURESELECT分析的Locatit。•吱吱作响专门解决了Locatit应用程序中的大型内存资源需求,并使用户能够处理与LoCatit相比的内存较少的数据。•尖叫还增加了一种可用于处理嵌合对齐的鲁棒算法,该嵌合对齐由读取段组成,这些片段在同一染色体或不同染色体上彼此之间彼此远远遥不可及。•尖叫报告的基本统计信息具有明确的定义,可以直接追溯到输出BAM文件中的读取。Statistics include: number of processed reads, number of reads passing the filter steps, number of correctly paired reads, number of read pairs flagged as duplicates in the input file, number of read pairs with at least one unmapped mate, number of chimeric read pairs, number of read pairs called as single consensus, number of read pairs called as duplex consensus, number of read pairs called as chimeric consensus, total duplicates确定的,失败的共识过滤器的读对数,读对的数量称为单个共识,在双工模式下质量过滤器失败。
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