2。How to Use this Product.....................................................................................................................5 2.1.Before you Begin................................................................................................................................................................................... 5 Sample Materials................................................................................................................................................................................... 5 Control反应............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Concentration.............................................................................................................................................................................. 5 Prevention of Carryover Contamination........................................................................................................................................ 5 2.2.Protocols................................................................................................................................................................................................... 6 LightCycler ® PRO and LightCycler ® 96 System protocols..................................................................................................... 6 Protocol for use with the LightCycler ® PRO System (Multiwell Plate 96 or 384).......................................................... 6 Protocol for use with the LightCycler ® 96 Instrument............................................................................................................. 7 Setup of the qPCR reaction for the LightCycler ® PRO and LightCycler ® 96 Instruments.......................................... 7 Two-Step RT-PCR.................................................................................................................................................................................. 8