2.1 Construction of recombinant plasmids ...........................................................................................9 2.2 Protein expression and purification .............................................................................................. 10 2.3 Electrophoretic characterization of proteins ............................................................................... 11 2.4 Electrical conversion ........................................................................................................................ 11 2.5 Flow cytometry sorting ................................................................................................................... 11 2.6 ELISA reader screening .................................................................................................................. 12 3.Directed evolution of GFP catenane ........................................................................................ 12
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