C.1 生物原料 ................................................................................................................................................ 4 C.2 流程图 .......................................................................................................................................................... 4 C.3 细胞的起源和来源 ........................................................................................................................................ 5 C.3.1 人类细胞 ................................................................................................................................................ 5 C.3.2 动物细胞 ................................................................................................................................................ 5 C.3.3 病毒/B 菌/其他来源 ................................................................................................................................ 6 C.3.4 微生物细胞 ................................................................................................................................................ 7 C.4 细胞历史 ................................................................................................................................................ 7 C.5 细胞基质的生成 ........................................................................................................................................ 8 C.6 细胞 B C.6.1 主细胞库 ...................................................................................................................................................... 10 C.6.2 工作细胞库 ...................................................................................................................................................... 10 C.6.3 原始细胞库 ...................................................................................................................................................... 11 C.6.4 二倍体细胞系 ...................................................................................................................................................... 11 C.6.5 肿瘤发生细胞系 ............................................................................................................................................. 12 C.7 细胞存库程序 ...................................................................................................................................................... 13 C.8 细胞库的特性分析和测试 ............................................................................................................................. 14 C.8.1 身份和纯度测试 ................................................................................................................................................. 15 C.8.2 细胞底物稳定性测试 ............................................................................................................................. 17 C.8.3 细胞核学和致瘤性测试 ............................................................................................................................. 19 C.8.4 致癌性测试 ............................................................................................................................................. 21 C.9 种子 ............................................................................................................................................................. 21 C.9.1 主种子 ............................................................................................................................................................. 23 C.9.2 工作种子 ............................................................................................................................................. 23 C.10 遗传构建体和重组细胞系 ............................................................................................................................. 24 C.10.1 宿主细胞 ............................................................................................................................................. 24 C.10.2 基因C.10.3 载体 ................................................................................................................................................................ 24 C.10.4 最终基因构建 ................................................................................................................................................ 24 C.10.5 重组细胞系的克隆和建立 ................................................................................................................................ 25 C.11 细胞生长和收获 ...................................................................................................................................................... 25 C.11.1 繁殖 ................................................................................................................................................................ 25 C.11.2 收获 ................................................................................................................................................................ 26 C.12 激活、纯化和下游加工 ................................................................................................................................ 26 C.12.1 激活 ................................................................................................................................................................ 27 C.12.2 净化...................................................................................................................................................... 28 C.12.3 稳定性处理 ...................................................................................................................................... 28 C.12.4 脱毒 ...................................................................................................................................................... 29