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DNA extraction → Whole genome sequencing RNA extraction → RNA sequencing Postoperative tumor tissue - Fresh frozen tissue ** 5 mm square x 1 HE stained preparation (for assessing tumor content ratio) DNA extraction → Whole genome sequencing RNA extraction → RNA sequencing Postoperative non-tumor tissue - Fresh frozen tissue ** 5 mm square x 1 HE stained preparation DNA提取→整个基因组测序RNA提取→RNA测序对体化学疗法血液 *20 mL/次血浆分离，DNA提取→CTDNA分析，整个基因组测序术前和术后血液** 20 mL/tims等离子体分离，DNA分离，DNA提取→CTDNA分析→CTDNA分析→CTDNA分析→CTDNA分析→CTDNA分析。协会Ariake医院・大阪大学医学院附属医院
JCOG2203A1
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