使用HSOP 461和HSOP 466(Spectratating GuideLine),使用T细胞的磁珠细胞分选 T细胞受体谱分型。 使用商业试剂盒(QIAGEN)的RNA提取以及遵循制造商的说明和内部标准操作程序HSOP499。 Reverse transcription for cDNA synthesis using commercial kit (Invitrogen) and in-house standard operating procedures HSOP 780 RT-PCR using HSOP 467 Fragment analysis HSOP 468 AutoMACS Pro (MiltenyiBiotec), GorScript TM First-Strand Synthesis System for RT-PCR (Promega), QiagenHot Star Taq DNA Polymerase, G Storm 4 PCR machine and Proflex机器,3500XL遗传分析仪(FSOP 491)。T细胞受体谱分型。使用商业试剂盒(QIAGEN)的RNA提取以及遵循制造商的说明和内部标准操作程序HSOP499。Reverse transcription for cDNA synthesis using commercial kit (Invitrogen) and in-house standard operating procedures HSOP 780 RT-PCR using HSOP 467 Fragment analysis HSOP 468 AutoMACS Pro (MiltenyiBiotec), GorScript TM First-Strand Synthesis System for RT-PCR (Promega), QiagenHot Star Taq DNA Polymerase, G Storm 4 PCR machine and Proflex机器,3500XL遗传分析仪(FSOP 491)。
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