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Figure 1: Flow diagram outlining factors contributing to increased generation of fast fashion associated garment waste (Gupta et al., 2022; Niinimäki et al., 2020; Sandin & Peters, 2018)......................................................................................................................................... 1 Figure 2: Stick representations of chromophore orientations.Chromoprotein SGBP(Cyan)(反式非链球菌)和荧光蛋白EQFP611(粉红色)(trans coplanar)和dsred(绿色)(CIS Coplanar dsred)。(Chiang et al., 2015)......................................................................................................................................... 5 Figure 3: Topologies related to type A, B and C CBMs.芳香族氨基酸产生CH-π相互作用的蓝色可视化,蓝色键 - 氨基酸地层以紫色可视化。Carbohydrate substrates visualised in green (Armenta et al., 2017.......................................................................................................................................... 8 Figure.使用Bradford试剂或灭绝系数(Ext COE)确定的结果。pH 7 bradford(a),pH 7寿命系数(b),pH 5.5 bradford(c),pH 5.5影合系数(d)。数据以多次比较的混合作用分析表示为生物重复物的平均值和标准偏差。*Significantly different (p < 0.05).........................................................................................................................................36 Figure 10: Percentage binding for each protein overvaried pH, temperature and incubation time.4:以下质粒的地图:PET-CHR AB(A),PET-CHR AB.CL(B),PET-CHR AB.CH2(C),PET-CHR SP(D),PET-CHR SP.CL(E),Petchr UM(f) (H)...........................................................................................................................................24 Figure 5: Absorbance spectra from 300 to 700 nm between pH 3 to 9 for the following proteins: AB (A), AB.Cl (B), AB.Ch2 (C), SP (D), SP.Cl(E), UM (F), UM.Cl (G) and UM.Ch2 (H)...........................................................................................................................................28 Figure 6: Absorbance spectra from 300 to 700 nm in 25 °C – 25 °C (in 5 °C increments) for the following proteins: (A) AeBue (AB), (B) AB.Cl, (C) AB.Ch2, (D) SP, (E) SP.Cl , (F) Ultramarine, (G) UM.Cl., (H) UM.Ch2...................................................................................................................................31 Figure 7: Protein standards and corresponding elution volume for Superdex 200 10/300 column.................................................................................................................................... 33 Figure 9在pH 5.5或7时,每种蛋白质的约束百分比结合百分比。室温过夜(RTON)(粉红色),室温超过2小时(RT2H)(蓝色),4°C过夜(4CON)(绿色)(绿色),4°C,超过2小时(4C2H)(紫色)。使用Bradford试剂(BRAD)或灭绝系数(Ext COE)确定的结果。UM.CH2(A - D),UM.CL(E - H)的结果。 数据以生物重复的平均值和标准偏差表示,并使用双向方差分析进行了多次比较。UM.CH2(A - D),UM.CL(E - H)的结果。数据以生物重复的平均值和标准偏差表示,并使用双向方差分析进行了多次比较。*Significantly different (p < 0.05).........................................................................................................................................40

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