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补充图5。低温促进混合DS/HT121- 6中的IM折叠。在（a）IC缓冲液和（b）从HELA细胞中获得的杂种-DS/HT121-6的1d 1 H NMR光谱的 Imino区域作为温度的函数（指示）。红色和灰色框分别突出显示针对Im-和dsDNA分析的Imino信号。Note that while the signal intensity pertinent to iM (highlighted in the red box) observed at 4 °C was essentially reduced to zero when the temperature was increased to 37 °C, the alterations in the NMR signal intensities (integral intensities – considering temperature- dependent line broadening and the inherently low signal-to-noise ratio in the in-cell NMR spectra) corresponding to the dsDNA segment (gray box) were最小。
补充信息内部NMR建议...
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