发酵完成后,有必要恢复所需的代谢物。最少,这将涉及将细胞与发酵肉汤分离。,但它也可能包括在有或没有细胞破坏的情况下纯化代谢产物的纯化;如果代谢物是细胞内的,则需要细胞破坏。此类操作称为下游处理。The steps involved in isolation of the desired microbial product are: (1) separation of cells from the fermented broth, (2) cell disruption if the product is intracellular or concentration of the broth if the product is extracellular (3) initial purification of the metabolite, (4) metabolite-specific purification in which the metabolite of interest is purified to a high degree, and (5) polishing of the metabolite (将其提高到98 -100%的纯度),在进一步浓缩并配制使用以供使用。