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Sequencing Method: in-house Genotypic antiretroviral resistance testingof HIV using Sanger sequencing (V7270) Nucleic acid extraction: BioMérieux EasyMag automated platform, with NucliSENS extraction reagents (as per manufacturer's instructions) Amplification: Bio-RAD Dyad DNA engine thermal cycler Gel electrophoresis and PCR cleanup using ThermoFisher exoSap-it（V7255）扩增子的定量：热泡量QUBIT 2.0荧光计（V7064）为抗病毒抗性测试（V7256）核酸序列数据制备Sanger测序反应
8825医疗单
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