Mixed lymphocyte reaction (MLR): CFSE-labelled PBMCs were co-cultured with different amount of research-grade GMP equivalent of vididencel (DCOne mDC) in the presence of AZA (5 µM) and/or VEN (5 µM) for 6 days and dilution of CFSE was analyzed as a measure of T cell proliferation using a flow cytometer.摄取测定:在AZA和/或VEN的情况下,在37°C下,将VPD450标记的PBMC与CFSE标记的Vididencel共培养3-4小时。使用流式细胞仪评估了PBMC中CFSE/VPD450-阳性人群的百分比。小鼠模型:用1x10 5人CD34+细胞接种雌性NSGS小鼠。允许植入和人类免疫细胞发育为12周的成熟期。随后,皮下植入了白血病细胞系DCONE DCONE LUC,并通过重量和嵌合物随机分配动物,并分配给以下组:未处理的对照,Vididencel(0.2x10 6; QWX2),AZA/Ven(AZA 1 mg/kg和Ven 50 mg/kg/kg/kg,qdx5)和vid+ aza+ az5和combista和combincination and aza/ven(aza/ven)和combinancian/combincination。监测肿瘤进展和治疗反应,以测量肿瘤体积。肿瘤进展和治疗反应,以测量肿瘤体积。
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