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(a) performing a targeted PCR amplification for more than 100 SNP loci on one or more chromosomes expected to be disomic in a single reaction mixture using more than 100 PCR primer pairs, wherein the reaction mixture comprises cell- free DNA extracted from a biological sample of a subject comprising DNA of mixed origin, wherein the DNA of mixed origin comprises DNA from the subject and DNA from a genetically distinct individual, wherein受试者和遗传上不同的个体都不是胎儿，其中混合起源的DNA包括来自移植的DNA，其中，放大的SNP基因座包括至少在1、2或3·'
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