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β-Mercaptoethanol PanReac-AppliChem A4338,0100 Sodium chloride (NaCl) PanReac-AppliChem 131659.1211 Tryptone Condalab 1612 Yeast Extract Condalab 1702 Bacteriological Agar Condalab 1800 Agarose D1 Medium EEO Condalab 8019 Liquid nitrogen n/a n/a Critical commercial assays NEBuilder® HiFi DNA Assembly Master Mix New England Biolabs E2621S Phusion TM High-fidelity DNA polymerase Thermo Fisher Scientific F530S MluI (10 U/µL) Thermo Fisher Scientific ER0561 BsaIHF®v2 (20 U/µL) New England Biolabs R3733S DNA Clean & Concentrator TM -5 Zymo研究D4004Nucleospin®质粒DNA纯化机构 - 纳格尔740588.250 Ribolock RNase抑制剂（40 u/μl）Thermo Fisher Scientific EO0381恢复TM逆转录（TM）逆转录酶Thero Fisher Fisher Fisher Scientific EP0441 Therus prolainsir prolapers themophirs dna Polymsisriast dna Polymsiss dna Prolymasse：003 3.003.003 3.003 3.003 3.003 3.003 3.003 3.003; 003 3.003 3.003 Nicotiana Benthamiana cas9（Bernabé-ortts等，2019）N/A寡核苷酸D2409 atttatattattAttCataCaatCaaAcc
DNA-DNA滑动摩擦和非平衡动力学在病毒基因组喷射和包装中的作用使用RNA
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