简介/背景:实验室的目的是向学生介绍如何通过质粒转化细菌来克隆感兴趣的基因。将要求学生设计一个实验,以通过确定存在哪些质粒中的三个基因中的哪些不同的质粒来区分三个不同的质粒。Key Concepts and Terms Covered: DNA, RNA, plasmid, vector, heritable information, genetic variation, antibiotic resistance, natural selection, gene transfer, enzyme Materials: per group: DNA Cloning video clip, DNA Cloning PowerPoint and note sheet, Online Lab Tutorials, Physical Lab activity - 2 transformation tubes, 1 packet of glass beads, 4 inoculating loops, 7 1ml-sterile transfer移液器,1条电线接种环,8种无菌培养皿,400毫升无菌LB琼脂,3毫升无菌氯化钙,3毫升无菌LB汤,4ml氨基霉素,4ml氨基霉素,4ml kanamycin,3 lb plates,3 lb plates,2 lb/kanamycin plate pg lb/ampimpim pg pg pgimm pgimm ppim plrein,质粒,200ul Pkan质粒,MM294倾斜培养(大肠杆菌),压碎冰和容器,42度厘米水浴,37度摄影孵化器,水浴,Bunsen燃烧器,废物容器,乙醇,乙醇。