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vi。纯化靶蛋白34 A.小规模分析34生长和诱导34诱导培养的光密度分析35总细胞蛋白（TCP）样本35培养基样品35周质分数样品 - 渗透性休克36可溶性细胞质分数37不溶于Preparation of Cell Extracts with BugBuster™ Protein Extraction Reagent 38 Soluble Fraction 38 Inclusion Body Purification 38 C. SDS-PAGE and Western Blot Analysis 39 Normalized SDS-PAGE Gel Loading 39 D. Large Scale Induction and Fractionation 40 Media Fraction 41 Periplasmic Fraction 41 Soluble Whole Cell Extract Fraction 41 Insoluble Whole Cell Extract Fraction–Isolation of Inclusion Bodies 42 Solubilization包含体和重折叠蛋白43亲和标签的纯化产品44
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