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• Data from a perfusion benchtop bioreactor scale show that Ala, Asn, His, Tyr, and Trp positively correlate with capsid production, while Gln correlates negatively • Analysis of cell culture media from shake flask experiments shows that Asn and other amino acids, including several essential amino acids, deplete during AAV production • Capsid titer per cell increases when Gln is absent from media • The REBEL device enabled通过准确，精确地定量氨基酸的数据驱动的RAAV生产的生物过程开发，用于HEK293细胞中的RAAV生产，从而进一步了解过程•正在进行更多的实验，以优化灌注过程，以获得全面的Capsids
优化基因治疗的可扩展RAAV生产
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