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• Persons living with HIV (PHIV) attending St Marys' hospital were consented and recruited between April and December 2023, all had: • Undetectable viral load at time of recruitment • Previous virological failure with drug resistance associated mutations (DRAMs) identified in ≥1 RNA sequence(s) (except 3 controls) • Virological data were extracted from clinical records and anonymized • 5ml EDTA blood was collected and用研究代码标记•从Buffy Coat细胞中提取DNA•通过经过验证的下一代测序（NGS）方法测序的蛋白酶蛋白酶修复转录酶（Prot-RT），其临界值15％•前病毒DNA序列与累积的历史RNA基因型和临床史及临床史
揭示HIV前病毒DNA测序的临床应用
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